Heterogeneity of Escherichia coli STb enterotoxin isolated from diseased pigs
نویسندگان
چکیده
منابع مشابه
Characterization of Escherichia coli strains producing heat-stable enterotoxin b (STb) isolated from humans with diarrhea.
Two of 49 cytolethal distending toxin-producing strains of Escherichia coli isolated from human stools contained the gene coding for heat-stable enterotoxin b (STb), as detected by a colony hybridization assay. The STb gene was found to be on a 70-kb plasmid also coding for heat-labile enterotoxin (pLT-I). Restriction endonuclease analysis showed the STb gene from human isolates to be similar t...
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We studied a collection of 79 colistin-resistant Escherichia coli isolates isolated from diseased pigs in France between 2009 and 2013. We determined a number of phenotypic and genetic characters using broth microdilution to characterize their antimicrobial susceptibility. We performed pulse field gel electrophoresis (PFGE) to assess their genetic diversity and assign them to phylogroups. High-...
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Fig 6. Effect of Zn -enrichment on claudin-1 displacement rate. (A) NP-40-soluble and (B) NP-40insoluble fractions. Gray: calcium-free medium, black: Zn-enriched medium, white: Zn-enriched medium treated with STb for 6, 12 and 24 h. Lower panel: Immunoblot showing claudin-1 and GAPDH used to evaluate their relative amounts. NP-40 cell extracted proteins were separated on a 12% acrylamide SDSPAG...
متن کاملEscherichia coli STb Enterotoxin Dislodges Claudin-1 from Epithelial Tight Junctions
Enterotoxigenic Escherichia coli produce various heat-labile and heat-stable enterotoxins. STb is a low molecular weight heat-resistant toxin responsible for diarrhea in farm animals, mainly young pigs. A previous study demonstrated that cells having internalized STb toxin induce epithelial barrier dysfunction through changes in tight junction (TJ) proteins. These modifications contribute proba...
متن کاملExpression of the cloned gene for enterotoxin STb of Escherichia coli.
This study involved the construction of hybrid plasmids to produce heat-stable enterotoxin type II of Escherichia coli (STb). The translation of the open reading frame for the STb gene estA was demonstrated in several ways. Studies using in vivo labeling with [35S]cysteine demonstrated a radiolabeled protein band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the expected molec...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2008
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.2008/000281-0